Lipidology: analysis of lipids

Lipidology is the scientific study of lipids.

Chromatography: to separate organic compounds from a mixture in gas state.
  • Is largely applied to asses cholesterol levels in animal and human fluids and tissues.
Autoradiogram of thin-layer chromatography of lipids from cells ...

Mass spectrometry: Lipidomics is made by the modality of  MS

Tutorial on lipidomics - ScienceDirect

Iodine number:
  • Carbon-carbon double bonds react with Iodine.
  • When it reacts, changes from a brownish color to a discoloured.
  • Iodine number=mass of iodine reactedmass of lipid×100
  • With the iodine number, there can be found the quantity of double bonds.
  • It measures the unsaturation level of a fat or oil.
Iodine numbers for different edible oils and fats. | Download Table


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